Professor Claus Yding Andersen from Copenhagen University Hospital reported the case to the medical journal Human Reproduction “This is the first time ever that a woman has two children in separate parts as a result of transplantation of cryopreserved ovarian tissue.

But more surprising is that the transplanted tissue continues working four years after the intervention, to the point that women, Stinnes bergholdt Holm, now must resort to contraception to avoid a new pregnancy.

These results confirm that cryopreservation of ovarian tissue is a valid method to preserve fertility and should encourage the development of this technique in clinical practice for girls and young women who face a treatment that could damage their ovaries.

In the world, including these two, there were only nine births as a result of autotransplantation of ovarian tissue previously frozen.

Danish Stinnes Holm Bergholdt, 32, gave birth to a daughter, Aviaja, in February 2007 after her ovarian transplant and fertility treatment. In September 2008 she is again pregnant naturally and had another daughter, Lucca.

Part of her right ovary was removed in 2004 and it was frozen before she was put to a chemotherapy treatment when she was diagnosed with Ewing’s sarcoma, a cancer that affects the bone or soft tissue.

Stinnes Holm Bergholdt had lost her other ovary for a benign tumor, years ago.

When she overcame cancer had an early menopause, so in December 2005, six samples of her own ovarian tissue were implanted on her.

Dr. Andersen says that “this treatment can be offered to any woman, it does not require any specialized center for collecting ovarian tissue samples,” and that “these are kept on ice for at least five hours, allowing enough time for transfer them to a laboratory equipped with tanks of liquid nitrogen.

He further explained that “with the removal of only one third of the ovary is able to maintain ovarian function for many years” and believes that “these tissues may be stored up to 40 years in liquid nitrogen.